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Peptide-like Polymers Exerting Effective Glioma-Targeted siRNA Delivery and Release for Therapeutic Application



·        BAG3;

·        glioma therapy;

·        glutathione triggered;

·        peptide-like polymer;

·        siRNA;

·        RNA delivery;

·        targeted therapy


Lipopolymer49, a solid-phase synthesized T-shaped peptide-like oligoamide containing twocentral oleic acids, 20 aminoethane, and two terminal cysteine units, isidentified as very potent and biocompatible small interfering RNA (siRNA)carrier for gene silencing in glioma cells. This carrier is combined with anovel targeting polymer 727, containing a precise sequence of Angiopep 2targeting peptide, linked with 28 monomer units of ethylene glycol, 40aminoethane, and two terminal cysteines in siRNA complex formation.Angiopep-polyethylene glycol (PEG)/siRNA polyplexes exhibit good nanoparticlefeatures, effective glioma-targeting siRNA delivery, and intracellular siRNArelease, resulting in an outstanding gene downregulation both in glioma cellsand upon intravenous delivery in glioma model nude mice without significantbiotoxicity. Therefore, this novel siRNA delivery system is expected to be apromising strategy for targeted and safe glioma therapy.


Application offluorescence melting curve analysis for dual DNA detection using single peptidenucleic acid probe



·        peptide nucleic acid;

·        fluorescence melting curveanalysis;

·        DNA analysis;

·        Genotyping


Peptide nucleic acid (PNA) is an artificially synthesized polymer.PNA oligomers show greater specificity in binding to complementary DNAs. Usingthis PNA, fluorescence melting curve analysis (FMCA) for dual detection wasestablished. Genomic DNA of Mycoplasma fermentans and Mycoplasma hyorhinis wasused as a template DNA model. By using one PNA probe, M. fermentans and M.hyorhinis could be detected and distinguished simultaneously in a single tube.The developed PNA probe is a dual-labeled probe with fluorescence and quencherdye. The PNA probe perfectly matches the M. fermentans 16s rRNA gene, with amelting temperature of 72°C. On the other hand, the developed PNA proberesulted in a mismatch with the 16s rRNA gene of M. hyorhinis, with a meltingtemperature of 44–45°C. The melting temperature of M. hyorhinis was 27–28°Clower than that of M. fermentans. Due to PNA's high specificity, this largermelting temperature gap is easy to create. FMCA using PNA offers an alternativemethod for specific DNA detection. © 2015 American Institute of ChemicalEngineers Biotechnol.Prog., 31:730–735, 2015