How to dissolve my peptide?

The solubility of peptides varies depending on its amino acid sequence and modifications. When we prepare it, the peptide is generally purified by HPLC using water and acetonitrile gradient. Here are some general tips for dissolving peptides:

• sonification will increase the solubility of peptides.
• For basic peptides, 10% acetic acid in the solvent will help dissolve peptides.
• For acidic peptides, 10% ammonium bicarbonate will help dissolve peptides
• For peptides with extremely low solubility in aqueous solutions, organic solvents should be used first (such as DMSO, isopropanol, methanol, acetonitrile). Once peptides are dissolved completely, water may be added slowly until the final concentration has been reached.